SRIKANTH LINGALA*, RAGHUNANDAN NERELLA, KIRAN POUDALA Isatins, Antimicrobial activity, Anti inflammatory activity, Amikacin, Amphoterecin 1152-1161 Volume 4, Issue 6 November - December 2013 In view of various biological activities and enormous importance of indoles, isatins and their derivatives, it was our interest to synthesize and characterize some new 5 - Sulfamoyl Isatin derivatives and evaluate them for antimicrobial and anti-inflammatory activity. An appropriate quantity of isatin hydrazone was heated under reflux with chloroacetyl chloride to give 2-Chloro-N- [(Z)-(2-oxo-5-sulfamoyl-indolin-3-ylidine) amino]acetamide which was then heated under reflux with various secondary amines to give 2-chloro-n-[(z)-(2-oxo-5 sulfamoyl -indolin-3-ylidene) amino] acetamide derivatives. The intermediates and final compounds were purified and their chemical structures have been confirmed by IR, 1H NMR, and Mass spectral data. All the synthesized compounds were screened for antibacterial activity against B. subtilis, B.cereus, S. epidermidis, S. typhi, P. aeruginosa and K. pneumoniae, antifungal activity against A.flavus, F.oxysporium and P. notatum and anti-inflammatory activity using carrageenan induced rat paw edema model. Most of the compounds tested have shown promising activities when compared with the standard drugs.