• ISSN : 2231 - 0541 (Online)
  • 0976 - 3090 (print)
  • NLM ID : 101613942
  • CAS CODEN : PHARN8
  • Frequency : Bi-Monthly

REVERSE PHASE HPLC METHOD FOR THE ANALYSIS OF DEFERASIROX IN BULK AND PHARMACEUTICAL FORMULATIONS

REVERSE PHASE HPLC METHOD FOR THE ANALYSIS OF DEFERASIROX IN BULK AND PHARMACEUTICAL FORMULATIONS

A rapid and sensitive high performance liquid chromatographic method is developed for the estimation of deferasirox in bulk and pharmaceutical formulations. Deferasirox was chromatographed on a reverse phase C8 column in a mobile phase consisting of phosphate buffer (pH 3.0 adjusted with orthophosphoric acid) and acetonitrile in the ratio 40:60 v/v. The mobile phase was pumped at a flow rate of 1 ml/min. with detection at 295 nm. The detector response was linear in the concentration of 10-125 ?g/ml. The limit of detection and limit of quantitation was found to be 0.01 and 0.05 ?g/ml, respectively. The intra and inter day variation was found to be less than 1%. The mean recovery of the drug from the solution containing 50 ?g/ml was 98.8%. The proposed method is simple, fast, accurate, precise and reproducible hence can be applied for routine quality control analysis of deferasirox in bulk and pharmaceutical formulations.