• ISSN : 2231 - 0541 (Online)
  • 0976 - 3090 (print)
  • NLM ID : 101613942
  • CAS CODEN : PHARN8
  • Frequency : Bi-Monthly

HIGH FAT / HIGH CARBOHYDRATE LONG TERM DIET ALTERED THE ANTIOXIDANT DEFENSE MECHANISM IN C57BL/6J MICE: EFFECT OF VANILLIC ACID ON LIPID PEROXIDATION MARKERS WITH ANTIOXIDANTS IN LIVER AND KIDNEY TISSUES

HIGH FAT / HIGH CARBOHYDRATE LONG TERM DIET ALTERED THE ANTIOXIDANT DEFENSE MECHANISM IN C57BL/6J MICE: EFFECT OF VANILLIC ACID ON LIPID PEROXIDATION MARKERS WITH ANTIOXIDANTS IN LIVER AND KIDNEY TISSUES

Recent studies have revealed that oxidative stress has been suggested as a contributory factor in development and complication of diabetes. The aim of the present study was to determine the protective effect of vanillic acid (VA) on lipid peroxidation and activities of antioxidant defense systems in high fat / high carbohydrate (HF/HC) diet induced C57BL/6J mice. The thirty six male C57BL/6J mice (18-22g) were used. Diabetic mice with hyperlipidemia were induced by oral administration of HF/HC diet on 18 weeks. Mice were divided into six groups of six mice in each cage. Group I served as normal mice intended for control; Group II normal with high dose of VA (40 mg/kg of body weight); Group III-VI receives HF/HC diet for 18 weeks, without VA for Group III and 10, 20 and 40 mg/kg of body weight of VA on last 8 weeks for Group IV, V and VI respectively. End of the study period glucose, insulin, lipid peroxidation markers, antioxidants, lipid profile and histopathological examination were analysed.Diabetic mice exhibited an increase in the levels of plasma glucose, lipid peroxidative markers and lipid profile; whereas, the activities of antioxidant weredecrease inliver and kidneys tissues as compared to control rats. Administration of VA to diabetic mice for last 8 weeks significantly inverted the damage associated with diabetes. These results indicated that VA may act as a safe preventive or therapeutic agent against diabetes mellitus.