• ISSN : 2231 - 0541 (Online)
  • 0976 - 3090 (print)
  • NLM ID : 101613942
  • CAS CODEN : PHARN8
  • Frequency : Bi-Monthly

ECO-FRIENDLY ANALYTICAL METHOD FOR QUANTITATIVE ESTIMATION OF HYDROCHLOROTHIAZIDE AND TELMISARTAN USING HYDROTROPIC SOLUBILIZING AGENTS

ECO-FRIENDLY ANALYTICAL METHOD FOR QUANTITATIVE ESTIMATION OF HYDROCHLOROTHIAZIDE AND TELMISARTAN USING HYDROTROPIC SOLUBILIZING AGENTS

In routine analysis of drugs, lots of organic solvents were used which are costlier, poisonous, and causing atmosphere contamination, so solubility enchantment of poorly water soluble by hydrotropic technique may be a proper choice to preclude the use of organic solvents so that eco-friendly, novel, safe, accurate and precise two methods has been developed for the simultaneous estimation of Hydrochlorothiazide and Telmisartan in tablet dosage form with 2 M Citric acid as hydrotropic solution. It was found that solubility enhanced of HCZ and TEL was more than 46 and 52 fold respectively in hydrotropic solution as compare against with distilled water. HCZ and TEL show maximum absorbances at 271.5 and 291 nm respectively. Citric acid solution did not show any absorbance above 240 nm and thus no interference in the estimation of drugs were seen. HCZ and TEL shows linearity in the concentration range of 5-25 ?g/ml and 10-50 ?g/ml (r2= 0.9999 and 0.9991). Method A simultaneous equation method employs 271.5 and 291 nm as two analytical wavelengths, method B is absorption ratio method, which uses 279.8 and 291 nm as two analytical wavelengths were used for estimation of HCZ and TEL. ICH guidelines were used to validated the developed methods. Results of accuracy, precision and other statistical validation analysis were found to be in excellent accordance with the prescribed values and therefore the both methods be able to used for routine monitoring of HCZ and TEL in industry in the assay of bulk drug and as well as tablets dosage form.