• ISSN : 2231 - 0541 (Online)
  • 0976 - 3090 (print)
  • NLM ID : 101613942
  • CAS CODEN : PHARN8
  • Frequency : Bi-Monthly

ASSAY OF GUANFACINE IN BULK AND ITS PHARMACEUTICAL FORMULATIONS BY EXTRACTION SPECTROPHOTOMETRY

ASSAY OF GUANFACINE IN BULK AND ITS PHARMACEUTICAL FORMULATIONS BY EXTRACTION SPECTROPHOTOMETRY

Three simple and sensitive spectrophotometric methods (A – C) for the assay of Guanfacine in pure and dosage forms based on the formation of chloroform soluble ion-associates under specified experimental conditions are described. Three acidic dyes, namely, wool fast Blue (WFB BL, method A), orange II (Tpooo, method B) and Naphthalene Blue 12 BR (NB-12BR, method C) are utilized. The extracts of the ion-associates exhibit absorption maxima at 590, 490 and 620 nm for methods A, B and C, respectively. Beer’s law and the precision and accuracy of the methods are checked by the UV reference method. The results are reproducible with an accuracy of ?1.0%. The methods are found to be suitable for the determination of Guanfacine in the presence of the other ingredients that are usually present in dosage forms.