• ISSN : 2231 - 0541 (Online)
  • 0976 - 3090 (print)
  • NLM ID : 101613942
  • CAS CODEN : PHARN8
  • Frequency : Bi-Monthly

ANTAGONISTIC ACTIVITY OF STREPTOMYCES SPECIES MPPO-02 ISOLATED FROM RHIZOSPHERE SOIL OF MAHISHI, KARNATAKA, INDIA

ANTAGONISTIC ACTIVITY OF STREPTOMYCES SPECIES MPPO-02 ISOLATED FROM RHIZOSPHERE SOIL OF MAHISHI, KARNATAKA, INDIA

Western Ghats of India are one of the global biodiversity hotspots. The present study was conducted to isolate and determine antibacterial activity of actinomycetes from a Western Ghat soil of Mahishi village, Thirthahalli Taluk, Shivamogga district, Karnataka. Out of 5 actinomycetes, one isolate designated MPPO-02 displayed marked antibacterial activity in primary screening by Cross streak technique. The isolate was identified as a species of Streptomyces based on microscopic and biochemical characteristics. In Agar well diffusion assay, the culture filtrate of MPPO-02 showed inhibitory activity against a panel of 10 bacteria. Gram positive bacteria were more susceptible than Gram negative bacteria to culture filtrate. Further studies on molecular characterization of the isolate MPPO-02 and purification of bioactive principle from culture filtrate are under progress. Further screening of soils of Mahishi can be fruitful in isolation of bioactive actinomycetes.