• ISSN : 2231 - 0541 (Online)
  • 0976 - 3090 (print)
  • NLM ID : 101613942
  • CAS CODEN : PHARN8
  • Frequency : Bi-Monthly

A VALIDATED RP- HPLC METHOD FOR THE ANALYSIS OF MOXIFLOXACIN HYDROCHLORIDE IN PHARMACEUTICAL DOSAGE FORMS

A VALIDATED RP- HPLC METHOD FOR THE ANALYSIS OF MOXIFLOXACIN HYDROCHLORIDE IN PHARMACEUTICAL DOSAGE FORMS

A simple and precise RP-HPLC method was developed and validated for the determination of Moxifloxacin
hydrochloride in pharmaceutical dosage forms. Chromatography was carried out on Shimadzu Item CBM-20A ,
control of LC-20A/10Avp/10A-Series solvent Delivery Module (Pump), SPD-M20A Photodiode Array Detector
using a mixture of Buffer: Methanol (55:45%) as the mobile phase at a flow rate of 1.0 mL min-1. The analyte was
monitored using UV detector at 293 nm. The Retention time of the drug is 5.816 min for Moxifloxacin. The
proposed method is found to be having linearity in the concentration range of 20-60? g mL-1 with correlation
coefficient of 0.999. The developed method has been statistically validated and found simple and accurate. The
mean recoveries obtained for Moxifloxacin HCL are in the range 99.3-102%. Due to its simplicity, rapidness, high
precision and accuracy of the proposed method it may be used for determining Moxifloxacin HCL in bulk and
dosage forms.